However, the development of these mesoscopic imaging systems is confounded by the scale-dependent geometric aberrations of optical elements ( 9). Recent progress, such as macroscopes ( 3), Mesolens microscope ( 5), two-photon mesoscope ( 6), RUSH ( 7), and COSMOS ( 8), are only beginning to bridge these scales. For example, perception and cognition arise from extended brain networks spanning millimeters to centimeters ( 3) yet rely on computations performed by individual neurons only a few micrometers in size ( 4). A major focus for recent technological developments is aimed at overcoming the barrier of scale ( 2). We further quantify the effects of scattering and background fluorescence on phantom experiments.įluorescence microscopy is an indispensable tool in fundamental biology and systems neuroscience ( 1). We experimentally validate the mesoscopic imaging capability on 3D fluorescent samples. Its expanded imaging capability is enabled by computational imaging that augments the optics by algorithms. The CM 2 features a compact lightweight design that integrates a microlens array for imaging and a light-emitting diode array for excitation. Here, we present a Computational Miniature Mesoscope (CM 2) that overcomes these bottlenecks and enables single-shot 3D imaging across an 8 mm by 7 mm field of view and 2.5-mm DOF, achieving 7-μm lateral resolution and better than 200-μm axial resolution. However, conventional microscopes/miniscopes are inherently constrained by their limited space-bandwidth product, shallow depth of field (DOF), and inability to resolve three-dimensional (3D) distributed emitters. ![]() The need for recording in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). Fluorescence microscopes are indispensable to biology and neuroscience.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |